Blood differential test

Definition

The blood differential test measures the percentage of each type of white blood cell (WBC) that you have in your blood. It also reveals if there are any abnormal or immature cells.

Alternative Names

Differential; Diff; White blood cell differential count

How the Test is Performed

A blood sample is needed.

A laboratory specialist takes a drop of blood from your sample and smears it onto a glass slide. The smear is stained with a special dye, which helps tell the difference between various types of white blood cells.

Five types of white blood cells, also called leukocytes, normally appear in the blood:

• Neutrophils
• Lymphocytes (B cells and T cells)
• Monocytes
• Eosinophils
• Basophils

A special machine or a health care provider counts the number of each type of cell. The test shows if the number of cells are in proper proportion with one another, and if there is more or less of one cell type.

How to Prepare for the Test

No special preparation is necessary.

How the Test will Feel

When the needle is inserted to draw blood, some people feel moderate pain. Others feel only a prick or stinging. Afterward, there may be some throbbing or slight bruising. This soon goes away.

Why the Test is Performed

This test is done to diagnose an infection, anemia, or leukemia. It may also be used to monitor one of these conditions or to see if treatment is working.

Normal Results

The different types of white blood cells are given as a percentage:

• Neutrophils: 40% to 60%
• Lymphocytes: 20% to 40%
• Monocytes: 2% to 8%
• Eosinophils: 1% to 4%
• Basophils: 0.5% to 1%
• Band (young neutrophil): 0% to 3%

What Abnormal Results Mean

Any infection or acute stress increases your number of white blood cells. High white blood cell counts may be due to inflammation, an immune response, or blood diseases such as leukemia.

It is important to realize that an abnormal increase in one type of white blood cell can cause a decrease in the percentage of other types of white blood cells.

An increased percentage of neutrophils may be due to:

• Acute infection
• Acute stress
• Eclampsia (seizures or coma in a pregnant woman)
• Gout (type of arthritis due to uric acid buildup in the blood)
• Acute or chronic forms of leukemia
• Myeloproliferative diseases
• Rheumatoid arthritis
• Rheumatic fever (disease due to an infection with group A streptococcus bacteria)
• Thyroiditis (a thyroid disease)
• Trauma
• Cigarette smoking

A decreased percentage of neutrophils may be due to:

• Aplastic anemia
• Chemotherapy
• Influenza (flu)
• Radiation therapy or exposure
• Viral infection
• Widespread severe bacterial infection

An increased percentage of lymphocytes may be due to:

• Chronic bacterial infection
• Infectious hepatitis (liver swelling and inflammation from bacteria or viruses)
• Infectious mononucleosis, or mono (viral infection that causes fever, sore throat, and swollen lymph glands)
• Lymphocytic leukemia (a type of blood cancer)
• Multiple myeloma (a type of blood cancer)
• Viral infection (such as mumps or measles)

A decreased percentage of lymphocytes may be due to:

• Chemotherapy
• HIV/AIDS infection
• Leukemia
• Radiation therapy or exposure
• Sepsis (severe, inflammatory response to bacteria or other germs)
• Steroid use

An increased percentage of monocytes may be due to:

• Chronic inflammatory disease
• Leukemia
• Parasitic infection
• Tuberculosis, or TB (bacterial infection that involves the lungs)
• Viral infection (for example, infectious mononucleosis, mumps, measles)

An increased percentage of eosinophils may be due to:

• Addison disease (adrenal glands do not produce enough hormones)
• Allergic reaction
• Cancer
• Chronic myelogenous leukemia
• Collagen vascular disease
• Hypereosinophilic syndromes
• Parasitic infection

An increased percentage of basophils may be due to:

• After splenectomy
• Allergic reaction
• Chronic myelogenous leukemia (a type of bone marrow cancer)
• Collagen vascular disease
• Myeloproliferative diseases (group of bone marrow diseases)
• Chickenpox

A decreased percentage of basophils may be due to:

• Acute infection
• Cancer
• Severe injury

Risks

There is little risk involved with having your blood taken. Veins and arteries vary in size from one person to another, and from one side of the body to the other. Taking blood from some people may be more difficult than from others.

Other risks associated with having blood drawn are slight, but may include:

• Excessive bleeding
• Fainting or feeling lightheaded
• Multiple punctures to locate veins
• Hematoma (blood accumulating under the skin)
• Infection (a slight risk any time the skin is broken)

References

Bain BJ. The peripheral blood smear. In: Goldman L, Schafer AI, eds. Goldman-Cecil Medicine. 26th ed. Philadelphia, PA: Elsevier; 2020:chap 148.

Chernecky CC, Berger BJ. Differential leukocyte count (diff) - peripheral blood. In: Chernecky CC, Berger BJ, eds. Laboratory Tests and Diagnostic Procedures. 6th ed. St Louis, MO: Elsevier Saunders; 2013:440-446.

Nasr MR, Hutchison RE. Leukocytic disorders. In: McPherson RA, Pincus MR, eds. Henry's Clinical Diagnosis and Management by Laboratory Methods. 24th ed. Philadelphia, PA: Elsevier; 2022:chap 34.